中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (8): 1216-1222.doi: 10.3969/j.issn.2095-4344.2015.08.013

• 组织工程血管材料 tissue-engineered vascular materials • 上一篇    下一篇

可吸收镁合金支架植入后犬冠状动脉C-反应蛋白、基质金属蛋白酶9和血管性血友病因子的表达

王汝朋,杨水祥   

  1. 首都医科大学附属北京世纪坛医院心内科,北京市 100038
  • 修回日期:2015-01-20 出版日期:2015-02-19 发布日期:2015-02-19
  • 通讯作者: 杨水祥,首都医科大学附属北京世纪坛医院心内科,北京市 100038
  • 作者简介:王汝朋,男,1986年生,山东省临沂市人,汉族,2012年北京大学医学部毕业,硕士,医师,主要从事冠心病的临床诊治研究。
  • 基金资助:

    国家863资助项目(2006AA02Z4Z3)

Effect of absorbable magnesium alloy stenting on expression of C-reactive protein, matrix metalloproteinase-9 and von Willebrand factor in dogs  

Wang Ru-peng, Yang Shui-xiang   

  1. Department of Cardiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • Revised:2015-01-20 Online:2015-02-19 Published:2015-02-19
  • Contact: Yang Shui-xiang, Department of Cardiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • About author:Wang Ru-peng, Master, Physician, Department of Cardiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • Supported by:

    the National High-Technology Research and Development Program of China (863 Program), No. 2006AA02Z4Z3

摘要:

背景:课题组前期研究从宏观角度验证了可吸收镁合金支架的生物安全性。

目的:观察可吸收镁合金支架植入后局部血管组织C-反应蛋白、基质金属蛋白酶9及血管性血友病因子的表达情况,从分子表达水平深层次探讨镁合金支架的组织相容性及安全性。
方法:将25枚可吸收镁合金支架植入25只防疫杂种犬冠状动脉前降支或左回旋支,未植入支架的5只犬冠状动脉作为正常对照组,支架植入后24 h、3 d、5 d、1周、1个月各处死动物5只,取支架植入后的血管标本做病理切片,应用免疫组织化学染色方法测定冠状动脉局部C-反应蛋白、基质金属蛋白酶9及血管性血友病因子的表达。
结果与结论:与正常对照组比较,支架植入后不同时间点冠状动脉局部C-反应蛋白、基质金属蛋白酶9及血管性血友病因子阳性细胞数明显增多(P < 0.01),A值显著升高(P < 0.01);与支架植入24 h、3 d、5 d、1周比较,植入1个月后冠状动脉局部C-反应蛋白、基质金属蛋白酶9及血管性血友病因子阳性细胞数明显减少(P < 0.01),A值显著降低(P < 0.01)。 结果表明可吸收镁合金支架植入后的组织炎症程度轻,持续时间短且无血栓形成,提示可吸收镁合金支架具有较好的组织相容性和安全性。

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 材料相容性, 支架植入, 炎症反应, 组织相容性, 动物实验, C-反应蛋白, 基质金属蛋白酶9, 血管性血友病因子

Abstract:

BACKGROUND: Our previous study have verified the biosafety of absorbable magnesium alloy stents from a macro perspective.

OBJECTIVE: To study the expressions of C-reactive protein, matrix metalloproteinase-9 and von Willebrand factor in local vascular tissue after magnesium alloy stenting, and to explore the histocompatibility and safety of magnesium alloy stents at the molecular expression level.
METHODS: Twenty-five absorbable magnesium alloy stents were implanted into the left anterior descending artery or circumflex artery of 25 epidemic prevention mongrel dogs. Five dogs with no stenting served as control group. Five dogs were sacrificed respectively at 24 hours, 3 days, 5 days, 1 week, 1 month after stenting, and vascular specimens were taken for preparation of pathological sections. The expressions of C-reactive protein, matrix metalloproteinase-9 and von Willebrand factor within the coronary artery wall were determined by immunohistochemical staining method.
RESULTS AND CONCLUSION: Compared with the control group, the number of cells positive for C-reactive protein, matrix metalloproteinase-9 and von Willebrand factor was significantly increased at different times after stenting (P < 0.01), as well as the mean absorbance value was increased greatly (P < 0.01). The number of cells positive for C-reactive protein, matrix metalloproteinase-9 and von Willebrand factor at 1 month after stenting was significantly lower than that at 24 hours, 3 days, 5 days and 1 week after stenting (P < 0.01), as well as the absorbance value was decreased significantly (P > 0.01). The inflammatory reactions induced by absorbable magnesium alloy stents are slight and last for short time, which suggests that the absorbable magnesium alloy stents have good histocompatibility and safety.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: Stents, Coronary Vessels, Histocompatibility

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